Rubus genomics research - Markers
The isolation of polymorphic codominant microsatellite markers in Rubus and in particular red raspberry, provides a tool for a number of applications including taxonomic classification, fingerprinting varieties, generation of genetic linkage maps (Graham et al. 2005a) and investigating gene flow between cultivated and wild raspberries (Graham et al. 2005). Microsatellite loci have been isolated by a number of methods including screening a PstI size selected genomic library with AC(13) and AG(13) (Graham et al. 2002, 2004) or by screening a nebulised genomic DNA library (Graham et al. 2005b) or by screening or sequencing from cDNA libraries (Graham et al. 2004). Positive clones identified through screening were sequenced and primer pairs designed to the sequences flanking identified SSRs. To facilitate PCR product identification on an automated DNA sequencer, one primer of each pair was fluorescently labelled.
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- SSR markers developed by screening form a nebulised DNA library
- SSR markers developed from PstI library
- Polymorphic SSR primer pairs developed from a Pst1 library of genomic DNA
- Polymorphic SSR primer pairs developed from cDNA
References
Graham J, Smith K, Woodhead M & Russell J (2002) Development and use of SSR markers in Rubus species. Molecular Ecology (2) 250-252.
Graham J, Smith K, MacKenzie K, Jorgenson L, Hackett C & Powell W (2004) The construction of a genetic linkage map of red raspberry (Rubus idaeus subsp. idaeus) based on AFLPs, genomic-SSR and EST-SSR markers. Theoretical and Applied Genetics 109:740-749.
