FruitBreeding - Rubus Genomics Markers - 1 | The James Hutton Institute

Rubus genomics research - Markers


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SSR markers developed by screening form a nebulised DNA library

Code Repeat Primers Expected size Alleles Latham Glen Moy  
Neb1A10 (GA)4-(GAGGA)2 L: ccgtcttcttcgtctcaacc
R: ccggaatcccaaactttctc
248 246, 252 x 252  
Neb1A21 (GCC)3-(GGCC)4 L: cctcggggtagacgatcac
R: caaaggcgtccctgttcc
159 159 x 161  
Neb1C15 (GTT)5-(GTT)3 L: ttctttcgaactcgctgagg
R: gctttcggaatagcaaaatcc
194 194 x 194  
Neb1D05 (GTT)5 L: agaaccacaagcaccaatcc
R: ccttgctctgtgcagtttga
172 172 x 172  
Neb1G02 (CT)9 L: tattggcgcttcgtctacct
R: gaagcagcaatctgcaacaa
250 250 x 250  
Neb1H15 (GAA)5 L: ccaccaagcttctccatttc
R: aacgcactttttgcagcttt
177 176, 196 x 176 Mapped
Neb1I07 (A)4(TC)2(A)7(G)2(A)3 L: ccccgaacacagcttacaac
R: caaagagtcgggtggaaaag
189 189 x 189  
Neb1J15 (CTT)5 L: ggtgcgcttcttggagaac
R: ctagcaccggaaccctagc
219 219 x 219  
Neb1K24 (AT)2(CAT)3 L: tcattcattgctttgtcatcg
R: taggcagcttcgacactgtt
204 204 x 204  
Neb1N07b (GT)7 -(CAGA)4 L: tgaagacccccttgatcttg
R: tcacgaatcctaagcacaaca
169 169 x 169  
Neb1O10 (T)10 L: ttgcaaaacacaccatgaaga
R: cgattggtgctattgtttactaaatg
173 173 x 173  
NebA09b (T)7-(GA) L: caaaagctgaacgtaccgaaa
R: ataagaagggtgccacatcg
230 231, 239 x 231 Mapped
NebA22 (T)12 L: gccgtccaatctaatcttgc
R: cgattgagtcgcagacagag
106 102 x 100, 104  
NebH24 (CA)2-(TA)4 L: gatgcggcagcttgataaac
R: cgattcaagctctagaattatcca
155 153, 155 x 151 Mapped
Norp4c8b (A)8-(GA)5 L: cgattaagagttgcagaagaagg
R: ccgtaatagcccaaccagaa
188 188, 192 x 188 Mapped
Norp2o23 (CT)8 L: gagaagacgtcagccaccag
R: ttgaagagaaagggaaggaaga
224 222, 224 x 224 Mapped