FruitBreeding - Rubus Genomics Markers - 2 | The James Hutton Institute

Rubus genomics research - Markers


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SSR markers developed from PstI library

Marker Repeat Primers Expected size Alleles Latham Glen Moy  
Rub5a (T)9 L: cccaggtatttcacctttgc
R: cccaaatttagcctcttatgga
155 155, 160 x 155 Mapped
Rub6b (GTA)5 L: ccaccgactttggtcgataa
R: aacaaaagcaaaaggtcaatcc
117 115 x 113, 115  
Rub17a (T)11 L: cctttgggactaggtttcca
R: gcattatcaccttcccttgg
147 145, 147 x 147, null  
Rub30a (T)13 L: agacagggccacgtgtgta
R: gggtgggagggaaatgatta
148 159 x 143  
Rub103a (T)12 L: tgcagattttggtggtgaag
R: ccattgcacaaaaccaatga
150 145,150 x 150 Mapped
Rub120a (CT)9-(AT)5 L: aagcccttctgcattcgata
R: ttgcaaattgaaattaggagca
189 181, 189 x 189  
Rub145a (gt)7 L: tgtcccagctttctggtttc
R: ggcatctgtgcggtaaaaat
131 131, null x 139, 146  
Rub149d1 (cat)3-(aat)5 L: cacacacacacagagcaagc
R:aaaaaggaaaggaaaagggttt
182 182, 190 x 182  
Rub177a1 (ct)6 L:ctcttcttcgcccagcaat
R: cattcccagctcacatagca
154 152 x 154  
Rub177a2 (ct)6 L: ctcttcttcgcccagcaat
R: cattcccagctcacatagca
154 195, 200 x 195, 200  
Rub220a (ga)11 L: gaaaatgcaaggcgaattgt
R: tccatcaccaacaccaccta
158 156,192 x 158, 163 Mapped
Rub212a (CT)21 L: gaaaaataggagaatgcttgtga
R: aaaactacatgcgacgcaaa
145 120, 141 x 145 Mapped
Rub214b (GA)13 L: atgagcttcttgccctgaaa
R: tcaagcaaaagtcccactcc
142 133, 170 x 133, 142 Mapped
Rub221a (tc)8 L: tggctgatgattctctgtcg
R: tctccattgccttcctcttc
161 161 x 161  
Rub222e (t)10-(a)11-(ga)15 L: gaggggcaattaaagggttt
R: tgttgtaatttggtttatccttgg
229 229, null x 214, null Mapped
Rub225a (taa)3-(tat)5 L: gagtgagagagggtgagtctgaa
R: aagctggacaccatacatcaaa
188 188 x 188  
Rub229a (tc)7 L: gtgtgccgttggtcatattg
R: aaagagcaggagcagagcag
149 168 x 149  
Rub 232b (ct)12 L: tgagcgagatgattggagtg
R: tatgtggtgatcatgcaagc
138 164 x 138, 142 Mapped
Rub236b (ga)9 L: tgtgccaagaactcatcgtc
R: ccgaacgcctccctactt
142 139,158 x 137, 139 Mapped
Rub238h (tgg)4 L: gtgacctccgagagcttgag
R: cttcaccgccactagtaccc
128 113, 138 x 127  
Rub244a (ctt)5 L: cccaaattgtacccatccac
R: aaacagcactgaagccaagc
156 147, null x 156, null  
Rub259b (ct)4-(ag)8 L: tggcacaagaagcctgtaac
R: tcccatatccctcagcattc
247 242, 250 x 247 Mapped
Rub265a (CT)7 L: agcacgtcagacgaaattga
R: aaaatttgacgacccgtttc
145 135, 154 x 145 Mapped
Rub274a (ct)5-(ct)4 L: cgatcacaaagcacgaaatg
R: cagccatagcccaaagagag
163 163 x 163  
Rub278b (ta)8-(gt)6 L: tgcctctctctatcgtctctctc
R: tggttcactttccatgttgtt
170 154, 164 x 170, null  
Rub284a (ctc)3-(tc)9 L: catggtccttgcaattcctt
R: ttatgtgccgctgatgtgtt
137 134, 161 x 137 Mapped