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src="http://www.hutton.ac.uk/sites/default/files/images/commercial/JHLimited_landscape_145pixels.jpg" alt="James Hutton Limited Logo" width="140" height="65" vspace="5" border="0" /></a></div> </div> <div id="banner"> <img src="images/fruitbreeding_banner.jpg" alt="FruitBreeding banner" /> </div> <div id="text"> <h1>Rubus genomics research - Markers</h1> <a href="RubusGenomicsMarkers.asp">Return to genomics markers page</a> <h3>Polymorphic SSR primer pairs developed from genomic DNA</h3> <table cellspacing="1" cellpadding="1" width="100%" summary="structural" border="1"> <colgroup><col align="left" width="5%"></col><col align="left" width="30%"></col><col align="left" width="35%"></col><col align="left" width="20"></col><col align="left" width="10"></col></colgroup> <tbody> <tr> <td>Code </td> <td>Repeat motif </td> <td>Primers </td> <td>Allele sizes Latham </td> <td>Allele sizes Glen Moy </td> </tr> <tr> <td>Rubus1b </td> <td>(ta)12-(ag)10 </td> <td>L: cctcttcaccgatttagacca R: tttagccccagtccaaaagtt </td> <td>212, 234 </td> <td>200, 229 </td> </tr> <tr> <td>Rubus2a </td> <td>(gt)12-g-(gt)8 </td> <td>L: tgagggaagaagaggcaaga R: cacgtgtgaccccaatgata </td> <td>175, 180 </td> <td>180, 188 </td> </tr> <tr> <td>Rubus4a </td> <td>(ct)14(ca)16 </td> <td>L: agcgaattgcatctctctctc R: gcactgaaaaatcatgcatctg </td> <td>143 </td> <td>139, 143 </td> </tr> <tr> <td>Rubus6a </td> <td>(ct)16(ca)32 </td> <td>L: tgcatgtgactttgcatctct R: gcactgaaaaatcatgcatctg </td> <td>139 </td> <td>139, 150 </td> </tr> <tr> <td>Rubus12a </td> <td>(ct)7(at)6(gt)10 </td> <td>L: attccccgcctcagaataat R: aaggtttgtgacgggaacag </td> <td>130, 150 </td> <td>141 </td> </tr> <tr> <td>Rubus16a </td> <td>(at)8(gt)11 </td> <td>L: tgttgtacgtgttgggcttt R: gggtgtttgccagtttcagt </td> <td>145, 155 </td> <td>145, 155 </td> </tr> <tr> <td>Rubusr19a </td> <td>(ta)5 </td> <td>L: gcagatcaatgaaagcccatt R: cggatcctccaaccttcat </td> <td>189, 199 </td> <td>201 </td> </tr> <tr> <td>Rubus20a </td> <td>(a)14 </td> <td>L: tgacataatgcatgaagggaaa R: cactatggttggccacagg </td> <td>139 </td> <td>150, 154 </td> </tr> <tr> <td>Rubus22a </td> <td>(at)16(gt)5 </td> <td>L: tgtggacgaccataacttgc R: tcggcatttatacacacacaca </td> <td>140, 150 </td> <td>150 </td> </tr> <tr> <td>Rubus24a </td> <td>(at)5 </td> <td>L: acacacgcacgtacagcact R: gcgcagtcaagtggactttt </td> <td>155, null </td> <td>166 </td> </tr> <tr> <td>Rubus25a </td> <td>(gt)8 </td> <td>L: gccaaacacaccgttatcttg R: cattaccacacgcttgatgc </td> <td>144, 150 </td> <td>144 </td> </tr> <tr> <td>Rubus26a </td> <td>(ct)11(ca)29 </td> <td>L: aacaccggcttctaaggtct R: gatcctggaaagcgatgaaa </td> <td>122, 150 </td> <td>150 </td> </tr> <tr> <td>Rubusr35a </td> <td>(ct)8 </td> <td>L: ttggaagcacaaaagcgata R: gcgacagccaaaacaaaagt </td> <td>210, 226 </td> <td>226 </td> </tr> <tr> <td>Rubusr43a </td> <td>(ct)5 </td> <td>L: tgcctaaagtttgctgctga R: tcgaatgtaactgcgagtgc </td> <td>201, 209 </td> <td>201 </td> </tr> <tr> <td>Rubus45c </td> <td>(t)10-(a)11-(ga)15 </td> <td>L: gaggggcaattaaagggttt R: tgttgtaatttggtttatccttgg </td> <td>215, 245 </td> <td>228, null </td> </tr> <tr> <td>Rubusr47a </td> <td>(ct)7-(ta)7 </td> <td>L: aagcaggacacctcagatgc R: cagccaaccatcatcagcta </td> <td>203, 244 </td> <td>203 </td> </tr> <tr> <td>Rubus49a </td> <td>(ta)7-(ga)7 </td> <td>L: cagccaaccatcatcagcta R: ttgttttcaggaggcaggac </td> <td>166, 182 </td> <td>166, 182 </td> </tr> <tr> <td>Rubusr56a </td> <td>(tg)12(ag)11 </td> <td>L: tggagattccaaataaacaaataccc R: tgtgtaaaccgttggatgaa </td> <td>212, 245 </td> <td>212 </td> </tr> <tr> <td>Rubus57a </td> <td>(ag)11 </td> <td>L: atgtgtgggggaagataacg R: tgtccccaacatttcatacaaa </td> <td>155 </td> <td>155, 185 </td> </tr> <tr> <td>Rubusr59b </td> <td>(ct)9 </td> <td>L: ctcctcctctttcctcgtca R: aagtgctgctgatgtgttgc </td> <td>279, 240 </td> <td>279 </td> </tr> <tr> <td>Rubusr76b </td> <td>(ct)5-(ct)4 </td> <td>L: ctcacccgaaatgttcaacc R: ggctaggccgaatgactaca </td> <td>250, 340 </td> <td>250, 340 </td> </tr> <tr> <td>Rubus98d </td> <td>(gaa)5-(ga)6 </td> <td>L: ggcttctcaatttgctgtgtc R: tgatttgaaatcgtgcggtta </td> <td>173, 178 </td> <td>178 </td> </tr> <tr> <td>Rubus102c </td> <td>(c )9(a)10 </td> <td>L: cccctcccctctctgtagat R: tcatgtgcaaacccgtacac </td> <td>95, 123 </td> <td>123 </td> </tr> <tr> <td>Rubus105b </td> <td>(ag)8 </td> <td>L: gaaaatgcaaggcgaattgt R: tccatcaccaacaccaccta </td> <td>158, 190 </td> <td>158, 164 </td> </tr> <tr> <td>Rubus107a </td> <td>(ag)8 </td> <td>L: gccagcaccaaaaacctaca R: tttcaccgtcaagaagaaagc </td> <td>155, 160 </td> <td>155 </td> </tr> <tr> <td>Rubus110a </td> <td>(tc)8 </td> <td>L: aaacaaaggataaagtgggaagg R: tgtcagttggagggagaaca </td> <td>180, 157 </td> <td>157 </td> </tr> <tr> <td>Rubus116a </td> <td>(ct)12-(t)10 </td> <td>L: ccaacccaaaaaccttcaac R: gttgtggcatggccttttat </td> <td>185, 190 </td> <td>193 </td> </tr> <tr> <td>Rubus117b </td> <td>(cata)6-(ga)8 </td> <td>L: ccaactgaaacctcatgcac R: acttggtcctgttggtctgg </td> <td>246, 286 </td> <td>240, 246 </td> </tr> <tr> <td>Rubus118b </td> <td>(ct)25 </td> <td>L: ccgcaaaacaaaaggtcaag R: ggattcttgccaaagtcgaa </td> <td>104, 112 </td> <td>137 </td> </tr> <tr> <td>Rubus119a </td> <td>(ga)8 </td> <td>L: gagcaaaacaaacacagatcaaa R: ctccaagtagtcacgcagca </td> <td>147, 150 </td> <td>147 </td> </tr> <tr> <td>Rubus123a </td> <td>(ag)8 </td> <td>L: cagcagctagcattttactgga R: gcactctccacccatttcat </td> <td>170, null </td> <td>140, 150 </td> </tr> <tr> <td>Rubus124a </td> <td>(at)9 </td> <td>L: atgagcgcgaaatgtggtat R: gtggaagttgttgtcgctca </td> <td>150, 162 </td> <td>150 </td> </tr> <tr> <td>Rubus126b </td> <td>(ct)31(ca)22 </td> <td>L: cctgcatttttctgtattttgg R: tcagttttcttcccacggtta </td> <td>150, 164 </td> <td>164, 201 </td> </tr> <tr> <td>Rubus137a </td> <td>(tg)8-(ta)4 </td> <td>L: tgtgagcagagtgaaggagcta R: agcattattcgcgcagtttt </td> <td>179, 187 </td> <td>179 </td> </tr> <tr> <td>Rubus145a </td> <td>(gt)7 </td> <td>L: tgtcccagctttctggtttc R: ggcatctgtgcggtaaaaat </td> <td>131 </td> <td>139, 142 </td> </tr> <tr> <td>Rubus153a </td> <td>(gt)11 </td> <td>L: cccagcttcagttggaaaga R: agaggctcatttgccttgaa </td> <td>154, 156 </td> <td>156 </td> </tr> <tr> <td>Rubus160a </td> <td>(ct)7 </td> <td>L: tccaactcggattctccatc R: tatgtgagctgggcatggt </td> <td>155, 160 </td> <td>155 </td> </tr> <tr> <td>Rubus163a </td> <td>(ga)35 </td> <td>L: tgttgtcctctgcaaccatt R: gcatagcccacaattagcaa </td> <td>203 </td> <td>203, 207 </td> </tr> <tr> <td>Rubus166b </td> <td>(tc)15 </td> <td>L: ccgcaagggttgtatcctaa R: gcatgagggcgatataaagg </td> <td>215, 221 </td> <td>219, 224 </td> </tr> <tr> <td>Rubus167a </td> <td>(tc)9 </td> <td>L: aaccctaagccaaggaccat R: caccacccatgacagtcaga </td> <td>166, 182 </td> <td>164, 180 </td> </tr> <tr> <td>Rubus194h </td> <td>(ga)12 </td> <td>L: tgtgttgttctctgcaacca R: agcccttacttttcctgcaa </td> <td>100, 111 </td> <td>111, 115 </td> </tr> <tr> <td>Rubus210a </td> <td>(ct)25 </td> <td>L: tcctgatggttgtctggttg R: ttcgaggcttttcagaaacaa </td> <td>101, 115 </td> <td>101, 115 </td> </tr> <tr> <td>Rubus223a </td> <td>(at)4-(ta)8-(at)10 </td> <td>L: tctcttgcatgttgagattctatt R: ttaaggcgtcgtggataagg </td> <td>146, 151 </td> <td>138 </td> </tr> <tr> <td>Rubus228a </td> <td>(ga)41 </td> <td>L: tggacagctttgtgcagagt R: gcttgcttgtatctccattgc </td> <td>118, 128 </td> <td>150 </td> </tr> <tr> <td>Rubus233a </td> <td>(ct)11 </td> <td>L: tgctgctttgttattttgtgc R: ggtcaacaatccttggataatca </td> <td>190, 200 </td> <td>190 </td> </tr> <tr> <td>Rubus237b </td> <td>((ttttc)3 </td> <td>L: catgcttgcatgatcaccac R: tgagccataaatttagagggatt </td> <td>134 </td> <td>136, 147 </td> </tr> <tr> <td>Rubus243a </td> <td>(ct)12 </td> <td>L: tgagcgagatgattggagtg R: tatgtggtgatcatgcaagc </td> <td>140 </td> <td>140, 174 </td> </tr> <tr> <td>Rubus251a </td> <td>(ga)10 </td> <td>L: gcatcagccattgaatttcc R: cccacctccattaccaactc </td> <td>157,183 </td> <td>157 </td> </tr> <tr> <td>Rubus252a </td> <td>(ta)7-(ag)7 </td> <td>L: cattggctacaggcaactca R: ttggcacaagtggacagaag </td> <td>128 </td> <td>128, 140 </td> </tr> <tr> <td>Rubus253a </td> <td>(ct)34(at)11 </td> <td>L: acctccaaatgccatagtgc R: caagaatctgatctcgtcttagca </td> <td>153, 165 </td> <td>153 </td> </tr> <tr> <td>Rubus256e </td> <td>(ctt)7(ct)8(at)10(ac)5 </td> <td>L: caacctgaaaaccaaactcg R: ctgagagcctgagaggtggt </td> <td>172, 211 </td> <td>141 </td> </tr> <tr> <td>Rubus257a </td> <td>(ct)4-(ct)6 </td> <td>L: ctcatcccaacaggtgtacg R: gagactccatggcgagaaag </td> <td>181, 185 </td> <td>158, 185 </td> </tr> <tr> <td>Rubus259f </td> <td>(ct)4-(ag)8 </td> <td>L: tggcacaagaagcctgtaac R: tcccatatccctcagcattc </td> <td>244, 252 </td> <td>247 </td> </tr> <tr> <td>Rubus260a </td> <td>(ga)13 </td> <td>L: ttcggaatttcggatcaaac R: gagagatctgacttgccaacg </td> <td>165, 173 </td> <td>147 </td> </tr> <tr> <td>Rubus262b </td> <td>(ag)15 </td> <td>L: tgcatgaaggcgatataaagg R: tccgcaagggttgtatccta </td> <td>217, 225 </td> <td>225 </td> </tr> <tr> <td>Rubus263f </td> <td>(at)16-(ca)4 </td> <td>L: attccgccctgcataaatc R: ggaaattggaaaccattgga </td> <td>241, 253 </td> <td>253 </td> </tr> <tr> <td>Rubus264b </td> <td>(ga)5-(gaaa)3(ga)7 </td> <td>L: tgcacagtttagggcaaaatc R: atcaggctgcatttttacgc </td> <td>175, 186 </td> <td>175, 186 </td> </tr> <tr> <td>Rubus268b </td> <td>(ga)10 </td> <td>L: ccaagacaatgacctgagca R: ggacagggttccacagagtg </td> <td>177, 183 </td> <td>183, 204 </td> </tr> <tr> <td>Rubus270a </td> <td>(ga)10 </td> <td>L: gcatcagccattgaatttcc R: cccacctccattaccaactc </td> <td>167, 185 </td> <td>158 </td> </tr> <tr> <td>Rubus275a </td> <td>(ag)27 </td> <td>L: cacaaccagtcccgagaaat R: catttcatccaaatgcaacc </td> <td>130 </td> <td>114, 145 </td> </tr> <tr> <td>Rubus277a </td> <td>(a)11(ag)8 </td> <td>L: gccccatcctgtacaaagaa R: ttgcaacaaaggtacgtaatgg </td> <td>234, 250 </td> <td>234 </td> </tr> <tr> <td>Rubus279a </td> <td>(ga)21 </td> <td>L: tcgacatggctagttctacacag R: ccccaacttaaaccattctca </td> <td>110, 132 </td> <td>120 </td> </tr> <tr> <td>Rubus280a </td> <td>(ag)13 </td> <td>L: ttcggaatttcggatcaaac R: cgaccaaaaaggaactcagc </td> <td>183, 191 </td> <td>165 </td> </tr> <tr> <td>Rubus285a </td> <td>(tc)9 </td> <td>L: tcgagaagcttgctatgctg R: ggatacctcaatggctttcttg </td> <td>167, null </td> <td>138 </td> </tr> <tr> <td>Rubus289a </td> <td>(ct)12 </td> <td>L: ttgcaccctagcatgtagca R: gaggtgaaaagggaacacga </td> <td>139 </td> <td>139, 166 </td> </tr> <tr> <td>Rubus293b </td> <td>(tc)9 </td> <td>L: atggccaatgaacctaccag R: tgttgatgtgttgccatattga </td> <td>180 </td> <td>159, 192 </td> </tr> </tbody> </table> </div> <div id="footer"> <table summary="structural table for site layout" width="100%"> <tr align="center"> <td> <a href="https://www.hutton.ac.uk/" target="new" title="The James Hutton Institute website - will open in a new window">The James Hutton Institute website</a> | <a href="https://www.huttonltd.com/" target="new" title="James Hutton Limited - will open in a new window">James Hutton Limited website</a> </td> </tr> <tr align="center"> <td>The James Hutton Institute Craigiebuckler, Aberdeen, AB15 8QH, Scotland | 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