FruitBreeding - Rubus Genomics Markers - 4 | The James Hutton Institute

Rubus genomics research - Markers


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Polymorphic SSR primer pairs developed from c-DNA

cDNA Repeat motif Primers Latham x Moy Homology (E value)
RubfruitE4 (ggttt)10 L: tcaaggctcctgctttaacc
R: gcagagttggagacatgaa
158, 160 x 160 AP004942(121/2e-24)
transcription factor
RubfruitE8 (ct)4(ta)4(ct)3 L: cataacctccagtggttcc
R: tgatgatgtcgatgcaggtt
180, 185 x 185 AF220491(214/1e-52)
o-methyltransferase
RubfruitG7 (ct)22 L: ggcacgagtcaagctctct
R: tggtttgtggttttgaagca
140, null x 159 no homology
RubfruitC1 ctt-(cct)7 L: cacgagcttcatcctcttcc
R: atccaaagcttttgcgattg
160, 170 x 160, 165 AY063105(133/5e-28)
ribosomal protein 1
Rubleaf86 (gt)9 L: ggaactttggagccacctac
R: tcggcaggttattcatttgt
235, 230 x 235 AJ131096(105/1e-19)
microsatellite
Rubleaf97 (ctt)-(cct)9 L: aacaaagctcctcgaccaga
R: tcgagatggtcagtccaaca
205, 210 x 205 ATCYC2B(139/6e-30)
cyclin 2b protein
Rubleaf107 (ta)10 L: gcggcaagctcatatactcc
R: ggcaagcttgaatgaatcct
210, 218 x 220 no homology
Rubleaf102 (cat-)16 L: gcacgagcctcatcatcttc
R: gatgttcgggccttttgtaa
230, 240 x 230 no homology